Coding

Part:BBa_K1943026:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-14)


TRPC5-20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2539
    Illegal BsaI.rc site found at 1627
    Illegal BsaI.rc site found at 2054
    Illegal SapI site found at 1722


Design Notes

This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.

Source

Original TRPC5:
Sequence from NCBI
Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline