Coding

Part:BBa_K1943026:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-14)


TRPC5-20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2539
    Illegal BsaI.rc site found at 1627
    Illegal BsaI.rc site found at 2054
    Illegal SapI site found at 1722


Design Notes

This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.

Source

Original TRPC5：
Sequence from NCBI
Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline